X-ray-induced apoptosis of BEL-7402 cell line enhanced by extremely low frequency electromagnetic field in vitro
✍ Scribed by Wen Jian; Zhao Wei; Cheng Zhiqiang; Fang Zheng
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 53 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0197-8462
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✦ Synopsis
Abstract
This study was designed to test whether extremely low frequency electromagnetic field (ELF‐EMF) could enhance the apoptosis‐induction effect of X‐ray radiotherapy on liver cancer cell line BEL‐7402 in vitro. EMF exposure was performed inside an energized solenoid coil. X‐ray irradiation was performed using a linear accelerator. Apoptosis rates of BEL‐7402 cells were analyzed using Annexin V‐Fit Apoptosis Detection kit. Apoptosis rates of EMF group and sham EMF group were compared when combined with X‐ray irradiation. Our results suggested that the apoptosis rate of BEL‐7402 cells exposed to low doses of X‐ray irradiation could be significantly increased by EMF. More EMF exposures obtain significantly higher apoptosis rates than fewer EMF exposures when combined with 2 Gy X‐ray irradiation. These findings suggested that ELF‐EMF could augment the cell apoptosis effects of low doses of X‐ray irradiation on BEL‐7402 cells in a synergistic and cumulative way. Bioelectromagnetics 30:163–165, 2009. © 2008 Wiley‐Liss, Inc.