X-ray absorption spectrometry as a tool for the study of molecular structure

✍ Scribed by W.E. Blumberg

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 123 KB
• Volume: 79
• Category: Article
• ISSN: 0020-1693
• DOI: 10.1016/s0020-1693(00)95051-5

✦ Synopsis

led to a narrower 67Zn NMR with a half-band width of 12 Hz. By adding 1 mM bovine insulin to the 50 miVf ZnClz solution, at pH 2.95, the half-band width of 67Zn NMR increased by three times. Addition of insulin more than 1 m&fled to a broader 67Zn NMR. The determined T1/T2 values of the 2M and 50 mM ZnClz solutions are almost unity. But, the T1/T2 ratio of 67Zn NMR of the Zn'+-insulin complex is not unity (1.36). From these findings, the correlation time, r,, and the quadrupole coupling constant are evaluated. The halfband widths of 67Zn NMR are temperature dependent, when Zn2' is bound to polymeric ligands. It is suggested from the temperature dependence that molecular weights of the ligands are closely correlated with the mechanism of the 67Zn NMR of the Zn2+ complexes. The relaxation mechanism on the 67Zn NMR will be discussed.