Working without Accumulation Membrane in Flow Fieldflow Fractionation. Effect of Sample Loading on Retention
✍ Scribed by Dora Melucci; Andrea Zattoni; Sonia Casolari; Matteo Reggiani; Ramses Sanz; Pierluigi Reschiglian; Giancarlo Torsi
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 155 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0003-4592
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✦ Synopsis
Abstract
Membraneless hyperlayer flow field‐flow fractionation (Hyp FlFFF) has shown improved performance with respect to Hyp FlFFF with membrane. The conditions for high recovery and recovery independent of sample loading in membraneless Hyp FlFFF have been previously determined. The effect of sample loading should be also investigated in order to optimize the form of the peaks for real samples. The effect of sample loading on peak retention parameters is of prime importance in applications such as the conversion of peaks into particle size distributions. In this paper, a systematic experimental work is performed in order to study the effect of sample loading on retention parameters. A procedure to regenerate the frit operating as accumulation wall is described. High reproducibility is obtained with low system conditioning time.