Wild boars as reservoirs of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli of different phylogenetic groups
✍ Scribed by Patrícia Poeta; Hajer Radhouani; Luís Pinto; António Martinho; Vítor Rego; Rogério Rodrigues; Alexandre Gonçalves; Jorge Rodrigues; Vanesa Estepa; Carmen Torres; Gilberto Igrejas
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 97 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
ESBL‐producing E. coli isolates have been isolated from eight of seventy seven faecal samples (10.4%) of wild boars in Portugal. The ESBL types identified by PCR and sequencing were bla~CTX‐M‐1~ (6 isolates) and bla~CTX‐M‐1~ + bla~TEM1‐b~ (2 isolates). Further resistance genes detected included tet (A) or tet (B) (in three tetracycline‐resistant isolates), aad A (in three streptomycin‐resistant isolates), cml A (in one chloramphenicol‐resistant isolate), sul 1 and/or sul 2 and/or sul 3 (in all sulfonamide‐resistant isolates). The intI 1 gene encoding class 1 integrase was detected in all ESBL‐producing E. coli isolates. One isolate also carried the intI 2 gene, encoding class 2 integrase. The ESBL‐producing E. coli isolates could be assigned to phylogenetic groups B1 (3 isolates), B2 (3 isolates) or A (2 isolates). Amino acid change in GyrA protein (Ser83Leu or Asp87Tyr) was detected in three nalidixic acid‐resistant and ciprofloxacin‐susceptible isolates. Two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC (Ser80Ile) were identified in two nalidixic acid‐ and ciprofloxacin‐resistant isolates. As evidenced by this study wild boars could be a reservoir of antimicrobial resistance genes. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)