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Widespread use of real-time PCR for rickettsial diagnosis

✍ Scribed by Aurélie Renvoisé; Jean-Marc Rolain; Cristina Socolovschi; Didier Raoult


Book ID
109309840
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
221 KB
Volume
64
Category
Article
ISSN
0928-8244

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Routine use of real-time quantitative PC
✍ Karen Brengel-Pesce; Patrice Morand; Anne Schmuck; Marie-Josette Bourgeat; Marly 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 191 KB 👁 1 views

## Abstract Real‐time quantitative polymerase chain reaction (PCR) on the LightCycler instrument (LC‐PCR) was developed to measure the Epstein‐Barr virus (EBV) load in clinical samples. LC‐PCR detected two copies of the EBV genome per 500 ng of DNA. Its specificity was confirmed by assays in EBV‐ne