Wide-field CARS-Microscopy : Functional Imaging at a Glimpse

✍ Scribed by M.A.M. Ritsch-Marte; S. Bernet; C. Heinrich

• Book ID: 102866613
• Publisher: Wiley (John Wiley & Sons)
• Year: 2007
• Weight: 664 KB
• Volume: 9
• Category: Article
• ISSN: 1439-4243
• DOI: 10.1002/imic.200790215

✦ Synopsis

Coherent anti-Stokes Raman scattering (CARS) microscopy is a branch of nonlinear microscopy that allows chemical imaging of targeted vibrational transitions in unstained samples. A resonantly enhanced blueshifted CARS signal is generated from NIR or visible light, thus the method is more sensitive than normal Raman microscopy and offers better resolution than IR microscopy. CARS microscopes are mostly set up as confocal scanning microscopes, but wide-field approaches are possible as well.