Whole-cell antigens of members of the sulfate-reducing genusDesulfovibrio

✍ Scribed by Rivers Singleton; Jean Denis; L. Leon Campbell

• Book ID: 104761381
• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 353 KB
• Volume: 141
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00408057

✦ Synopsis

Antisera have been developed against the wholecell antigens of Desulfovibrio afrieanus Benghazi and Walvis Bay, D. vulgaris Hildenborough, D. salexigens British Guiana, D. gigas, and D. desulfurieans Essex 6. An enzymelinked immunoadsorption assay (ELISA) was developed to measure the reaction of these antisera with the homologous and heterologous antigens. The ELISA method demonstrated a reaction between pre-immune sera and cells of D. africanus, D. gigas and D. desulfurieans, suggesting the presence of a lectin-like substance on these cell surfaces. Extensive cross-reactions were seen between the antisera and heterologous cells, suggesting the sharing of a number of surface antigens amongst the Desulfovibrio. However, the pattern of these cross-reactions was different from that observed for an ELISA reaction developed for the cytochrome c3 from various Desulfovibrio.