Whole-body section fluorescence imaging – a novel method for tissue distribution studies of fluorescent substances
✍ Scribed by Siver Moestue; Paula Nunez; Andrew Healey; Roger Bjerke; Bård Indrevoll; Tore Skotland; Svein Olaf Hustvedt
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 327 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1555-4309
- DOI
- 10.1002/cmmi.264
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✦ Synopsis
Abstract
The present study demonstrates the usefulness of whole body section fluorescence imaging, a novel technique used in optical imaging drug discovery. This method is in principle an analog of whole body autoradiography, except that fluorescence is measured instead of radioactivity. The method was shown to have a linear concentration–response relationship over a 1000‐fold concentration range. Densitometric image analysis allowed semiquantitative studies of drug disposition and selective tumor retention of an optical imaging drug candidate. Copyright © 2009 John Wiley & Sons, Ltd.