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Whole blood assay of glucose-6-phosphate dehydrogenase: potential for simplified immunoassay

โœ Scribed by Sock Ying Tham; Jacqueline E Pearson; John W Kane; Paul H Treloar; Pankaj M Vadgama


Book ID
108469991
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
95 KB
Volume
50
Category
Article
ISSN
0925-4005

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We developed an assay for serum magnesium (Mg) by coupling phosphoglucomutase (EC 2.7.5.1) with glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The kinetic generation of NADPH by the action of the above two enzymes upon glucose-l-phosphate (Gl-P) and glucoso-l,6-diphosphate (G-1,6-P) was proportion