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Wet oxidation pre-treatment of woody yard waste: parameter optimization and enzymatic digestibility for ethanol production

✍ Scribed by Geert Lissens; Helene Klinke; Willy Verstraete; Birgitte Ahring; Anne Belinda Thomsen


Publisher
Wiley (John Wiley & Sons)
Year
2004
Tongue
English
Weight
129 KB
Volume
79
Category
Article
ISSN
0268-2575

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✦ Synopsis


Abstract

Woody yard waste with high lignin content (22% of dry matter (DM)) was subjected to wet oxidation pre‐treatment for subsequent enzymatic conversion and fermentation. The effects of temperature (185–200 °C), oxygen pressure (3–12 bar) and addition of sodium carbonate (0–3.3 g per 100 g DM biomass) on enzymatic cellulose and hemicellulose (xylan) convertibility were studied. The enzymatic cellulose conversion was highest after wet oxidation for 15 min at 185 °C with addition of 12 bars of oxygen and 3.3 g Na~2~CO~3~ per 100 g waste. At 25 FPU (filter paper unit) cellulase g^−1^ DM added, 58–67% and 80–83% of the cellulose and hemicellulose contained in the waste were converted into monomeric sugars. The cellulose conversion efficiency during a simultaneous saccharification and fermentation (SSF) assay at 10% DM was 79% for the highest enzyme loading (25 FPU g^−1^ DM) while 69% conversion efficiency was still reached at 15 FPU g^−1^ DM. Total carbohydrate recoveries were high (91–100% for cellulose and 72–100% for hemicellulose) and up to 49% of the original lignin and 79% of the hemicellulose could be solubilized during wet oxidation treatment and converted into carboxylic acids mainly (total carboxylic acids = 3.1–7.4% on DM basis). Copyright © 2004 Society of Chemical Industry