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Weak acids, weak bases, and intracellular pH

โœ Scribed by Albert Roos


Book ID
118970932
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
284 KB
Volume
33
Category
Article
ISSN
0034-5687

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We describe a new method for calibrating intracellular pH (pH,) measurements by flow cytometry, based on the null point method proposed originally by Eisner et al. (Pflugers Arch 413:55+558, 1989). The method involves suspending cells loaded with pH-sensitive dyes, such as SNARF-1 or BCECF, in deh