Flow cytometric calibration of intracell
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Sue Chow; David Hedley; Ian Tannock
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Article
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1996
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John Wiley and Sons
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English
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We describe a new method for calibrating intracellular pH (pH,) measurements by flow cytometry, based on the null point method proposed originally by Eisner et al. (Pflugers Arch 413:55+558, 1989). The method involves suspending cells loaded with pH-sensitive dyes, such as SNARF-1 or BCECF, in deh