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Water solubilization of xanthene dyes by post-synthetic sulfonation in organic media

✍ Scribed by Anthony Romieu; Delphine Tavernier-Lohr; Stéphane Pellet-Rostaing; Marc Lemaire; Pierre-Yves Renard


Publisher
Elsevier Science
Year
2010
Tongue
French
Weight
379 KB
Volume
51
Category
Article
ISSN
0040-4039

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✦ Synopsis


Highly water-soluble fluorescent fluorescein and rhodamine dyes were synthesized through amidification of their carboxylic acid functionality with original di-or tri-sulfonated amino linkers derived from taurine or a-sulfo-b-alanine. This post-synthetic derivatization was performed in organic media both to minimize the premature hydrolysis and to suppress the precipitation of the involved active ester of fluorophore, frequently encountered using standard Schotten-Baumann conditions.