✦ LIBER ✦
Watching Moving Molecules in Real Time : Fluorescence Lifetime Imaging in Frequency-Domain Widefield Microscopes
✍ Scribed by Glen Redford; Karl Kilborn
- Book ID
- 102866567
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2006
- Weight
- 579 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1439-4243
No coin nor oath required. For personal study only.
✦ Synopsis
Along the domain of spectroscopy and biophysics labs, fluorescence lifetime is rapidly becoming an accessible tool for "mainstream" molecular biology research involving live cell imaging. In measuring a different physical property of a fluorophore, fluorescence lifetime imaging (FLI) has advantages in measuring protein proximity, polymerisation, relative concentration of different molecules, separation of different labels with spectral overlap, ion concentration, and removal of autofluorescence.