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Wall coating for capillary electrophoresis on microchips

✍ Scribed by Vladislav Dolník

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
386 KB
Volume
25
Category
Article
ISSN
0173-0835

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✦ Synopsis

Abstract

This review article with 116 references describes recent developments in the preparation of wall coatings for capillary electrophoresis (CE) on a microchip. It deals with both dynamic and permanent coatings and concentrates on the most frequently used microchip materials including glass, poly(methyl methacrylate), poly(dimethyl siloxane), polycarbonate, and poly(ethylene terephthalate glycol). Characterization of the channel surface by measuring electroosmotic mobility and water contact angle of the surface is included as well. The utility of the microchips with coated channels is demonstrated by examples of CE separations on these chips.

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In capillary electrophoresis, covering the inner capillary surface with a coating is an efficient way to minimize both the electroosmotic flow and sorption of w analytes on the capillary wall. We modified the procedure by Cobb et al. Anal. Ž .x Chem. 62, 2478 1990 for preparing wall coating to perm