Wacław Szybalski's contribution to immunotherapy: HGPRT mutation & HAT selection as first steps to gene therapy and hybrid techniques in mammalian cells

Roots. Use of the HPRT gene and the HAT

Roots. Use of the HPRT gene and the HAT selection technique in DNA-mediated transformation of mammalian cells: First steps toward developing hybridoma techniques and gene therapy

✍ Waclaw Szybalski 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 803 KB

## Abstract In 1956, I decided to apply my experience in microbial genetics to developing analogous systems for human cell lines, including the selection of mutants with either a loss or gain of a biochemical function. For instance, mutants resistant to azahypoxanthine showed a loss of the HPRT enz