Volume changes of cells in solutions containing both penetrating and non-penetrating solutes, and their relation to the ?permeability ratio?
✍ Scribed by Jacobs, M. H.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1933
- Tongue
- English
- Weight
- 466 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0095-9898
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✦ Synopsis
An earlier paper (Jacobs, '33 b) has dealt with the nature of the theoretical volume changes that, subject to certain simplifying assumptions, would occur in a cell placed in an isosmotic solution of a penetrating non-electrolyte. The present paper treats in much the same way the more complicated case of a cell exposed to a solution containing, in any desired concentrations, both penetrating and non-penetrating substances. This case is of considerable practical importance, since it is involved in the simplest and in some other respects the best osmotic method at present available for the measurement of permeability constants of living cells for both water and penetrating solutes. This method as described by the author (Jacobs, '33 a) consists in placing the cell to be studied in its natural medium, to which has been added the penetrating substance in any chosen amount, and in measuring the shrinkage followed by the return to the initial volume which occurs in such a solution. From a knowledge of the initial and the minimum volumes and the time of attainment of the latter, it is possible by the use of a prepared chart to obtain the values of the two permeability constants. It has been shown that the values of these constants as determined by 121 JOURNAL OX" CELLULAR AND COMPARATIVE PHYSIOLOOY, YOL. 3, NO. 2