Volume changes accompanying collagen denaturation
β Scribed by R. G. Christensen; J. M. Cassel
- Book ID
- 101714810
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1967
- Tongue
- English
- Weight
- 247 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
Synopsis
A dilatometric technique is employed to measure the volume changes occurring on denaturation of collagen solutions and tendons. Partial specific volumes of tropocollagen are calculated to be 0.686 ml./g. in water and 0.689 ml./g. in 0.1M citrate buffer. From data on non-aqueous solutions, it is estimated that most of the volume change arise? from changes in polymer configiiration rather than from changes in solvation.
π SIMILAR VOLUMES
## Abstract The change in apparent molal volume Ο of DNA on thermal denaturation in carbonate buffer at pH 11.0 has been determined by the dilatometric method. It was found that Ο increases sigmoidally during the helixβcoil transition. Several methods, including a colorimetric technique that closel
Dilatometric measurements were made to determine the change in apparent specific volume (p of DNA reaulting from thermal denaturation in neutral solution. (p increased continuously with temperature in the range 10-85Β°C. No deviations from a monotonically rising curve were observed in the (p versus t