Volatile anesthetics affect the morphology of rat glioma C6 cells via RhoA, ERK, and Akt activation
✍ Scribed by Piet W.L. Tas; Stepan Gambaryan; Norbert Roewer
- Book ID
- 102300455
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 290 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0730-2312
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Treatment of rat glioma C6 cells with the β‐receptor agonist isoproterenol induces a massive increase in cAMP. Concomitantly the cells change their morphology from a fibroblast‐type to an astrocyte‐like (stellated) cell shape. The stellated morphology can be completely reverted by thrombin and sphingosine‐1‐phosphate (S‐1‐P) but also to a certain extent by clinical concentrations of volatile anesthetics. The anesthetic‐induced reversion of the stellated cell shape seems to be mediated by a number of cellular alterations. Central to the effect is most likely a RhoA/Rho‐kinase activation, but also the MAPKK/MEK and the Akt/protein kinase B pathway are activated by the anesthetics. With the use of specific inhibitors we were able to show that activation of the MAPKK/MEK pathway inhibits, whereas activation of the Akt/protein kinase B pathway stimulates the reversal of the stellated cell shape by the anesthetics. In summary, volatile anesthetics affect the morphology of rat glioma C6 cells by activation of the RhoA/Rho kinase, the MAPKK/MEK, and the Akt/protein kinase B signaling pathways. J. Cell. Biochem. 102: 368–376, 2007. © 2007 Wiley‐Liss, Inc.