Vitellogenin levels in allatectomized female crickets, Gryllus bimaculatus de geer (ensifera, gryllidae)

✍ Scribed by Klaus H. Hoffmann; Dorian Sorge

• Book ID: 102661521
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 595 KB
• Volume: 32
• Category: Article
• ISSN: 0739-4462
• DOI: 10.1002/(sici)1520-6327(1996)32:3/4<549::aid-arch26>3.0.co;2-b

✦ Synopsis

Allatectomy of cricket nymphs up to 24 h after the moult to the last instar resulted in moulting to normal adults which laid a low number of eggs. No juvenile hormone Ill and no vitellogenin were detected in the haemolymph of allatectomized females by means of GC-mass spectrometry (MS) and rocket immunoelectrophoresis, respectively, whereas the eggs contained the normal pattern of vitellins as judged by SDS-PAGE. Ovariectomy of previously allatectomized females led to a moderate accumulation of vitellogenin in the haemolymph. Administration of the juvenile hormone analogues hydroprene, methoprene, and fenoxycarb to allatectomized females restored egg production to a variable extent. Injections of 20-hydroxyecdysone increased haemolymph vitellogenin titer of allatectomized females, but did not affect the number of produced eggs. Treatment with hydroprene and 20-hydroxyecdysone showed a synergistic effect. It is suggested that juvenile hormone does not have an all-embracing control on vitellogenesis, though it does exert a marked quantitative effect on vitellogenin synthesis and the rate of egg production.