Vitelline coat of Unio elongatulus: II. Biochemical properties of the 220- and 180-kD components

✍ Scribed by R. Focarelli; A. Santucci; R. Lampariello; Dr. Floriana Rosati

• Book ID: 102951621
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 570 KB
• Volume: 40
• Category: Article
• ISSN: 1040-452X
• DOI: 10.1002/mrd.1080400115

✦ Synopsis

In a previous study we found that two glycoproteins with apparent molecular weights of 220 kD and 180 kD account for 80-90% of the material dissolved from the vitelline coat of the egg of the bivalve mollusk, Unio elongatulus (Focarelli and Rosati, 1993 Mol Reprod Dev 35:44-51). In this study we isolated and purified these glycoproteins by electroelution. The two proteins differ in many respects: the 180-kD molecule is acidic in nature and highly heterogeneous, whereas the 220-kD protein is neutral and less heterogeneous. Both seem to have 0and N-linked oligosaccharide chains. The 180-kD protein contains 13-16% carbohydrate, whereas the 220-kD molecular contains only 7-8%. Amino acid analysis and peptide mapping also show that each protein represents a unique polypeptide chain. o 1995 Wiley-Liss. Inc.