Vitamin A uptake by human skin in vitro

✍ Scribed by H. Törmä; A. Vahlquist

• Book ID: 104764248
• Publisher: Springer-Verlag
• Year: 1984
• Tongue: English
• Weight: 551 KB
• Volume: 276
• Category: Article
• ISSN: 0340-3696
• DOI: 10.1007/bf00413360

✦ Synopsis

Results are presented establishing that epidermis accumulates vitamin A from serum retinolbinding protein (RBP). Strips of human breast skin (0.2-0.3-ram thick) were incubated in a serum-flee medium. From the rate of glucose oxidation, the tissue was viable for at least 48 h at 32~ in 5% CO2 air.

[3H]-Retinol-RBP (10-6 M) was added to the medium for 1-24 h, after which epidermis and dermis were split and separately extracted with hexane after saponification. [3H]-Retinol was isolated by high performance liquid chromatography (HPLC). Epidermis had 6-7 times higher affinity for [3H]-retinol than dermis. The uptake could be saturated by substrate and was inhibited with unlabelled retinol-RBP but not with serum albumin. Furthermore, although the uptake was temperature-dependent, it seemed independent of cellular energy production. The epidermal accumulation of [3H]-retinol was reduced by the filtering action of dermis. On the basis of these observations, an in vitro model for the delivery of vitamin A to human skin has been proposed.