Visualizing Inhibition of Nucleosome Mobility and Transcription by Cisplatin-DNA Interstrand Crosslinks in Live Mammalian Cells

Rapid qualitative evaluation of DNA tran

Rapid qualitative evaluation of DNA transcription factor NF-κB by microchip electrophoretic mobility shift assay in mammalian cells

✍ Sonoko Inoue; Noritada Kaji; Masatoshi Kataoka; Yasuo Shinohara; Yukihiro Okamot 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 207 KB

## Abstract We have developed a separation technique for DNA–protein complex based on electrophoretic mobility shift assay (EMSA) by microchip electrophoresis, which we call microchip electrophoretic mobility shift assay (μEMSA). To evaluate the μEMSA, we employed recombinant human nuclear factor‐κ