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Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

✍ Scribed by Spira, Felix; Dominguez-Escobar, Julia; Müller, Nikola; Wedlich-Söldner, Roland

Book ID
115530879
Publisher
MyJoVE Corporation
Year
2012
Tongue
English
Weight
379 KB
Volume
63
Category
Article
ISSN
1940-087X

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## Abstract In modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epi‐illumination modes. In wide‐field imaging, scattering of highly coherent laser light due to imperfections in the light path typically leads to nonuniform il