𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Visualization of chromosomes in mitotically arrested cells of the fission yeastSchizosaccharomyces pombe

✍ Scribed by K. Umesono; Y. Hiraoka; T. Toda; M. Yanagida

Publisher
Springer-Verlag
Year
1983
Tongue
English
Weight
495 KB
Volume
7
Category
Article
ISSN
0172-8083

No coin nor oath required. For personal study only.

✦ Synopsis

Three sets of mitotic chromosomes were observed in wild type or cdc mutants (nda3-KM311 and nda2-KM52) of the fission yeast S. pombe by the DAPI staining method. The block of microtubular functions by thiabendazole or by the mutations caused their individual appearance in mitotically arrested cells. The chromosomes have a characteristic size; the length ratio of short, medium and long ones was roughly t:2:3, consistent with the previous genetical data ( Kohli et al. 1977). Double staining with ethidium bromide and DAPI showed that the nucleolus was always associated with the shortest chromosome. Pairlike structures resembling sister chromatids were also seen.

πŸ“œ SIMILAR VOLUMES

A revised chromosome map of the fission
A revised chromosome map of the fission yeastSchizosaccharomyces pombe
✍ Anthony Gygax; Pierre Thuriaux πŸ“‚ Article πŸ“… 1984 πŸ› Springer-Verlag 🌐 English βš– 661 KB

The genetic map of the nuclear genome of the fission yeast Schizosaccharomyces pombe has been extended by mitotic and meiotic mapping data. A total of 158 markers are now assigned to the three linkage groups known in this organism, and 118 of them have been located on the corresponding chromosome ma

Genetic analysis of antisuppressor mutan
Genetic analysis of antisuppressor mutants in the fission yeastSchizosaccharomyces pombe
✍ P. Thuriaux; M. Minet; F. Hofer; U. Leupold πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 708 KB

Fourteen unlinked sin genes could be mutated to recessive antisuppressor alleles preventing the expression of suppressors in the fission yeast Schizosaccharomyces pombe. cyh1 alleles, resistant to the ribosomal inhibitor cycloheximide, also have some antisuppressor effect. The genetical and physiolo

Genetic studies of purine breakdown in t
Genetic studies of purine breakdown in the fission yeastSchizosaccharomyces pombe
✍ James R. Kinghorn; Rudolf Fluri πŸ“‚ Article πŸ“… 1984 πŸ› Springer-Verlag 🌐 English βš– 547 KB

Purines such as hypoxanthine, xanthine, uric acid, allantoin and allantoic acid serve as sole nitrogen sources for the yeast Schizosaccharomyces pombe. A number of classes of mutants unable to use purines have been isolated and genetically analysed. Mutants in the urol gene lack uricase, alll lack a

Organisation of the complex locustrp1in
Organisation of the complex locustrp1in the fission yeastSchizosaccharomyces pombe
✍ Pierre Thuriaux; Wolf-Dietrich Heyer; AndrΓ© Strauss πŸ“‚ Article πŸ“… 1982 πŸ› Springer-Verlag 🌐 English βš– 601 KB

65 trp1(-) alleles of Schizosaccharomyces pombe have been analysed for their interallelic complementation pattern, suppressibility by nonsense suppressors and position of the corresponding mutation site on an intragenic map of the trp1 locus. In addition to the three complementation classes previous