𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Visualising the dissociation of sequence selective ligands from individual binding sites on DNA

✍ Scribed by Michael C. Fletcher; Keith R. Fox


Book ID
115932029
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
515 KB
Volume
380
Category
Article
ISSN
0014-5793

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Selection of High-Affinity Binding Sites
✍ S. Pierrou; S. Enerback; P. Carlsson πŸ“‚ Article πŸ“… 1995 πŸ› Elsevier Science 🌐 English βš– 695 KB

We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble