โ Scribed by Curtis A. Machida
No coin nor oath required. For personal study only.
Researchers from academia and biotechnology describe proven molecular methods for the construction, development, and use of virus vectors for gene transfer and gene therapy. Offering detailed step-by-step instructions to ensure successful results, these experts detail the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Additional chapters demonstrate the use of virus vectors in the brain and central nervous system. Comprehensive and highly practical, Viral Vectors for Gene Therapy: Methods and Protocols provides not only researchers with the basic tools needed to design targeted gene delivery vectors, but also clinicians with an understanding of how to apply viral vectors to the treatment of genetic disorders.
Cover......Page 1
Preface......Page 6
Contents......Page 8
1 Use of the Herpes Simplex Viral Genometo Construct Gene Therapy Vectors......Page 17
2 Construction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous System......Page 49
3 Improved HSV-1 Amplicon Packaging System Using ICP27-Deleted, Oversized HSV-1 BAC DNA......Page 67
4 Herpes Simplex Amplicon Vectors......Page 77
5 Strategies to Adapt Adenoviral Vectors for Targeted Delivery......Page 105
6 Use of Recombinant Adenovirus for Gene Transfer into the Rat Brain......Page 129
7 Generation of Adenovirus Vectors Devoid of All Viral Genes by Recombination Between Inverted Repeats......Page 151
8 Packaging Cell Lines for Generating Replication-Defective and Gutted Adenoviral Vectors......Page 169
9 Improving the Transcriptional Regulation of Genes Delivered by Adenovirus Vectors......Page 183
10 Targeted Integration by Adeno-Associated Virus......Page 217
11 Development and Optimization of Adeno-Associated Virus Vector Transfer into the Central Nervous System......Page 237
12 A Method for Helper Virus-Free Production of Adeno-Associated Virus Vectors......Page 253
13 Novel Tools for Production and Purifi cation of Recombinant Adeno-Associated Viral Vectors......Page 271
14 Recombinant Adeno-Associated Viral Vector Types 4 and 5......Page 285
15 Trans-Splicing Vectors Expand the Packaging Limits of Adeno-Associated Virus for Gene Therapy Applications......Page 303
16 Generation of Retroviral Packaging and Producer Cell Lines for Large-Scale Vector Production with Improved Safety and Titer......Page 325
17 An Ecdysone-Inducible Expression System for Use with Retroviruses......Page 347
18 In Vivo Infection of Mice by Replication-Competent MLV-Based Retroviral Vectors......Page 359
19 Development of Simian Retroviral Vectors for Gene Delivery......Page 369
20 Self-Inactivating Lentiviral Vectors and a Sensitive Cre-loxP Reporter System......Page 383
21 Lentiviral Vectors for Gene Transfer to the Central Nervous System......Page 399
22 A Highly Effi cient Gene Delivery System Derived from Feline Immunodefi ciency Virus (FIV)......Page 421
23A Multigene Lentiviral Vector System Based on Differential Splicing......Page 449
24 Production of Trans-Lentiviral Vector with Predictable Safety......Page 465
25 Human Immunodefi ciency Virus Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells......Page 483
26 Semliki Forest Virus Vectors for Gene Transfer......Page 509
27 Semliki Forest Virus (SFV) Vectors in Neurobiology and Gene Therapy......Page 519
28 Semliki Forest Virus Vectors for Large-Scale Production of Recombinant Proteins......Page 541
29 Development of Foamy Virus Vectors......Page 561
30 Poxviral/Retroviral Chimeric Vectors Allow Cytoplasmic Production of Transducing Defective Retroviral Particles......Page 581
Index......Page 595
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