𝔖 Bobbio Scriptorium
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Viral specificity of hepatitis E virus antigens identified by fluorescent antibody assay using recombinant HEV proteins

✍ Scribed by Michael A. Purdy; Dorrie Carson; Karen A. McCaustland; Daniel W. Bradley; Michael J. Beach; Kris Krawczynski

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
351 KB
Volume
44
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

A fluorescent antibody (FA) assay for hepatitis E virus antigen (HEVAg) in infected liver tissue was used to confirm the presence of virus‐specific antigens in hepatocytes during the course of infection. With the cloning of the HEV genome it is now possible to determine which viral antigens are recognized by this FA assay. Recombinant HEV proteins covering the carboxyl half of HEV open reading frame 2 (ORF2) were used in this study to demonstrate that some of the most immunoreactive virus‐specific antigens detected by FA are contained within this region of ORF2 (nucleotides 6169–7126).

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✍ Favorov, M. O.; Khudyakov, Y. E.; Mast, E. E.; Yashina, T. L.; Shapiro, C. N.; K 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 926 KB

To develop an enzyme immunoassay (EIA) for IgM antibody t o hepatitis E virus (HEW (IgM anti-HEV) and IgG antibody t o HEV (IgG anti-HEV), a synthetic gene encoding several liner immunodominant antigenic epitopes f r o m HEV structural proteins was assembled as a chimeric recombinant mosaic protein