Viral P21 Ki-RAS protein: A potent intracellular mitogen that stimulates adenylate cyclase activity in early G1 phase of cultured rat cells
✍ Scribed by Douglas J. Franks; James F. Whitfield; Jon P. Durkin
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 428 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Rat kidney (NRK) cells infected with a temperature-sensitive mutant of the Kirsten sarcoma virus were arrested in the Go/G1 phase of their cell cycle by incubation in serum-deficient medium at a p21-inactivating temperature of 41 "C. These quiescent ts K-NRK cells were then stimulated to transit GI and initiate DNA replication by lowering the temperature to 36"C, which rapidly reactivated p21.
Reactivating the viral Ki-RAS protein by temperature shift led to an increase in adenylate cyclase activity in early GI phase. The Ki-RAS protein increased the sensitivity of adenylate cyclase to guanyl nucleotides by a mechanism that seemed to involve inactivation of the enzyme's inhibitory GI regulatory protein.