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Viral DNA contamination is responsible for Epstein–Barr virus detection in cytotoxic T lymphocytes stimulated in vitro with Epstein–Barr virus B-lymphoblastoid cell line

✍ Scribed by Mathilde Berthomé; Géraldine Gallot; Régine Vivien; Béatrice Clémenceau; Jean-Michel Nguyen; Marianne Coste-Burel; Henri Vié

Publisher: Springer-Verlag
Year: 2010
Tongue: English
Weight: 331 KB
Volume: 59
Category: Article
ISSN: 0340-7004
DOI: 10.1007/s00262-010-0913-2

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## Abstract Unfractionated mononuclear (UM) cells and T cells freshly prepared from the blood of adult donors were co‐cultivated in microtest plate wells with progressively lower numbers of cells from the autologous EB‐virus‐transformed B‐cell line. The fresh cells present in co‐cultures from EB vi