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Vibrational Spectra of Blue-Membrane Bacteriorhodopsin: Picosecond Resonance Coherent Anti-Stokes Raman Spectroscopy

โœ Scribed by Edgar Ligon; Laszlo Ujj; Olaf Weidlich; Andreas Popp; G. H. Atkinson


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
468 KB
Volume
28
Category
Article
ISSN
0377-0486

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โœฆ Synopsis


Vibrational spectra of bacteriorhodopsin (BR) in its deionized form (blue-membrane BR) were measured via picosecond resonance coherent anti-Stokes Raman scattering (PR/CARS). More than 18 vibrational bands in the 750-1650 cm-1 region not previously assigned were observed. Comparisons of these PR/CARS spectra with vibrational data recorded from BR species containing either an all-trans (i.e. BR-570) or 13-cis retinal (e.g. BR-548 found in dark-adapted BR and the picosecond BR intermediate K-590) were used to characterise more fully isomeric forms of the retinal chromophore found in blue-membrane BR.


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