Vibrational Spectra of Blue-Membrane Bacteriorhodopsin: Picosecond Resonance Coherent Anti-Stokes Raman Spectroscopy
โ Scribed by Edgar Ligon; Laszlo Ujj; Olaf Weidlich; Andreas Popp; G. H. Atkinson
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 468 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0377-0486
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โฆ Synopsis
Vibrational spectra of bacteriorhodopsin (BR) in its deionized form (blue-membrane BR) were measured via picosecond resonance coherent anti-Stokes Raman scattering (PR/CARS). More than 18 vibrational bands in the 750-1650 cm-1 region not previously assigned were observed. Comparisons of these PR/CARS spectra with vibrational data recorded from BR species containing either an all-trans (i.e. BR-570) or 13-cis retinal (e.g. BR-548 found in dark-adapted BR and the picosecond BR intermediate K-590) were used to characterise more fully isomeric forms of the retinal chromophore found in blue-membrane BR.
๐ SIMILAR VOLUMES
A novel approach to the preparation and monitoring of molecules in high vibrational levels in their electronic ground state is presented. An ultrashort laser pulse prepares a well defined wavepacket in the electronic excited state and, following the evolution of this wavepacket, the proper frequency