Vibrational Raman Optical Activity of α-Lactalbumin: Comparison with Lysozyme, and Evidence for Native Tertiary Folds in Molten Globule States

Raman optical activity characterization

Raman optical activity characterization of native and molten globule states of equine lysozyme: Comparison with hen lysozyme and bovine α-lactalbumin

✍ Ewan W. Blanch; Ludmilla A. Morozova-Roche; Lutz Hecht; Wim Noppe; Laurence D. B 📂 Article 📅 2000 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 297 KB

Vibrational Raman optical activity (ROA) spectra of the calcium-binding lysozyme from equine milk in native and nonnative states are measured and compared with those of the homologous proteins hen egg white lysozyme and bovine ␣-lactalbumin. The ROA spectrum of holo equine lysozyme at pH 4.6 and 22°