Vibrational Circular Dichroism Spectra of Proteins in the Amide III Region: Measurement and Correlation of Bandshape to Secondary Structure
✍ Scribed by Bernoli I. Baello; Petr Pancoska; Timothy A. Keiderling
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 197 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Fourier-transform infrared (FT-ir), Raman, Vibrational circular dichroism (VCD) spectra have and vibrational CD (VCD) with secondary structure been measured for 23 globular proteins dissolved in (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Each approach has strengths and weaknesses H 2 O/phosphate buffer over the 1400 to 1100 cm 01 region which have been reviewed separately (7). In particular, which encompasses the amide III mode. Spectral rewith regard to the technique that is the focus of this sponses characteristic of the dominant secondary paper, we have earlier shown that VCD has an enstructure type were found as broad features at Ç1300 hanced sensitivity to secondary structure, has the abilcm 01 , with the extreme forms having positive VCD for ity to provide data for several presumably independent highly helical proteins and negative VCD for highly vibrational transitions, and yields spectra for the amsheet-containing proteins. Quantitative correlation ide I and II modes whose bandshapes can be quantitawith secondary structure was carried out using pretively correlated to secondary structure (14-16, 20viously developed factor analysis and restricted multi-23). These latter correlations show improved sheet preple regression (FA/RMR) techniques. Since the abdiction but worse helix prediction than obtained under sorbance intensity of the amide III mode is difficult comparable conditions using other techniques such as to determine due to overlap with other transitions, an ECD (4,16). VCD over the amide I and II modes also alternative, absolute intensity-independent, simple yields somewhat better overall structural predictions structural analysis method was used. A linear regresthan with FT-ir bandshapes (20). Both VCD-and FTsion was developed between the fractional components ir-based predictions are improved by combining those of secondary structure for the protein set and the overdata sets with ECD data as input to the correlation lap integrals of the normalized spectra from the set scheme (11, 13, 16, 20) but have little improvement