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Viability of vitrified mouse embryos using various cryoprotectant mixtures

✍ Scribed by H. Ishimori; Y. Takahashi; H. Kanagawa


Book ID
118379334
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
365 KB
Volume
37
Category
Article
ISSN
0093-691X

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Eight-cell mouse embryos were frozen by using glycerol, ethylene glycol, or erythritol as the cryoprotectant. The samples were slowly cooled (0.5"C/min) to temperatures between -10 and -79Β°C before direct transfer to liquid nitrogen (-196Β°C) and thawed slowly ( -20"C/min) or rapidly ( -500Β°C/min). T