Viability of, and transfer of a plasmid from, E. coli K12 in the human intestine
β Scribed by ANDERSON, E. S.
- Book ID
- 109695581
- Publisher
- Nature Publishing Group
- Year
- 1975
- Tongue
- English
- Weight
- 315 KB
- Volume
- 255
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/255502a0
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π SIMILAR VOLUMES
The effects of eight different polA alleles on the replication of six different non-transferring enterobacterial plasmids have been tested. Using phage P1CM transduction, different allelic polA mutations were introduced into E. coli K12 strains carrying one of several antibiotic resistance plasmids.
Using Mu/mini-Mu mixed lysates prepared by induction of Salmonella typhimurium and Klebsiella pneumoniae lysogenic for Mucts62 or the Mu-P1 hybrid, MPh1, and mini-Mu, Mu18A-1, we were able to transfer Arg and His genes from Klebsiella and Salmonella into E. coli K12.