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Vesicular transport of myelin proteolipid and cerebroside sulfates to the myelin membrane

✍ Scribed by M. C. Brown; M. Besio Moreno; E. R. Bongarzone; P. D. Cohen; E. F. Soto; Dr. J. M. Pasquini

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 719 KB
Volume: 35
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490350407

No coin nor oath required. For personal study only.

✦ Synopsis

The possibility that cerebroside sulfates and myelin proteolipid (PLP) could be simultaneously located in transport vesicles destined to be assembled in myelin was investigated in the brain of 20 day old rats. The brain was homogenized and fractionated according to Burkart et al. (J B i d Chem 257:3151-3156, 1982) to obtain a microsomal fraction that was further subfractionated in a linear sucrose density gradient following the procedure of Siegrist et al. (J Neurochern 33:497-504, 1979) to obtain a vesicular fraction which has been shown to transport cerebroside sulfates (Burkart et al., as above)

. This fraction was associated with acid bydrolase activity and bad a lipid composition different from that of myelin and microsomal fractions. Studied by slab gel electropboresis, dot blot, and Western blot analysis, using a highly specific anti-PLP antibody, it was found to contain myelin PLP. In view of previous findings of several laboratories including our own, the presence of myelin proteolipid in a vesicular fraction which is related to the transport of cerebroside sulfates gives further support to the hypothesis that the delivery of both constituents to the myelin membrane could be associated.

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