Very high isotope incorporation in the C-1 position of glucose by exchange with deuterium or tritium gas
✍ Scribed by Mervyn A. Long; Hiromi Morimoto; Philip G. Williams
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- French
- Weight
- 609 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
The experimental conditions which control the exchange from deuterium or tritium gas into the C‐1 proton position of glucose in aqueous solution have been studied in detail, with a view to determining the factors which maximize exchange into glucose, but minimize exchange into the solvent water. The favoured conditions for producing glucose with close to 100% isotope labelling in the C‐1 position, and with negligible formation of labelled byproducts, were a reaction time of 6 to 8 hours, a temperature of 60 °C, and a pH of 7 or higher. Pd/BaSO~4~ was the preferred catalyst and slow pre‐addition of the deuterium or tritium gas to the catalyst bed was essential to maximize the subsequent isotope exchange into the substrate.