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Versatile gene cassette plasmids to facilitate the construction of generalized and specialized cloning vectors

✍ Scribed by Mongkolsuk, Skorn; Vattanaviboon, Paiboon; Rabibhadana, Siritida; Kiatpapan, Pornpimon


Book ID
122103300
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
184 KB
Volume
124
Category
Article
ISSN
0378-1119

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A shuttle cosmid vector, pANsCos1, has been constructed for Escherichia coli and filamentous fungi. This vector contains two cos sequences separated by a single XbaI restriction site. pANsCos1 allows the efficient construction of representative genomic libraries from as little as 15-20 micrograms of