✦ LIBER ✦

Verapamil preferentially potentiates in-vitro cytotoxicity of vincristine on malignant lymphoid cells

✍ Scribed by Dr Imtiaz A. Malik; Nicolas V. Costea

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 484 KB
Volume: 10
Category: Article
ISSN: 0278-0232
DOI: 10.1002/hon.2900100314

No coin nor oath required. For personal study only.

✦ Synopsis

Verapamil has been shown to overcome acquired drug resistance to vincristine in P388 leukemia both in vitro and in vivo. To study the selectivity of this action, the effect of addition of verapamil on the cytotoxicity of vincristine was studied using lymphocytes from eight patients with chronic lymphocytic leukemia (CLL), lymphoblasts from a T-acute lymphoblastic leukemia (T-ALL) cell line (GM 3639), and peripheral blood lymphocytes (PBL) from eight normal healthy volunteers. Using the differential staining cytotoxicity (DiSC) assay, we demonstrated that verapamil at 1 p~ concentration potentiated the in-vitro cytotoxicity of vincristine on CLL and GM 3639 cells in concentrations of 0-04-0.25 pg/l. There was however, no enhancement of cytotoxicity noted against the control PBL. The data demonstrate that verapamil preferentially enhances the in-virro cytotoxicity of vincristine on CLL and GM 3639 cells but no enhancement of cytotoxicity is seen against PBL.

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