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Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads

โœ Scribed by Herbert P Schweizer


Book ID
104361587
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
83 KB
Volume
12
Category
Article
ISSN
0958-1669

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โœฆ Synopsis


Improved tools for Pseudomonas research include small, broad-host-range vectors that allow regulated expression from the lac operon and T7 promoters whose biology is well understood and adaptable to many bacteria. To facilitate studies on gene regulation, tracking and monitoring of bacteria in diverse environments, and the construction of biosensors, various reporter genes with versatile assay formats have been developed that can be delivered on plasmid, transposon and integration-proficient vectors.


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Plasmids related to the broad host range
โœ Gary Ditta; Thomas Schmidhauser; Emanuel Yakobson; Peter Lu; Xiao-Wu Liang; Debo ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 373 KB

Derivatives of plasmid pRK290 that are useful for cloning and for analyzing gene expression in a wide variety of Gram-negative bacteria are described. A smaller broad host range plasmid derived from RK2, with properties similar to that of pRK290, is also described.