Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads
โ Scribed by Herbert P Schweizer
- Book ID
- 104361587
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 83 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
โฆ Synopsis
Improved tools for Pseudomonas research include small, broad-host-range vectors that allow regulated expression from the lac operon and T7 promoters whose biology is well understood and adaptable to many bacteria. To facilitate studies on gene regulation, tracking and monitoring of bacteria in diverse environments, and the construction of biosensors, various reporter genes with versatile assay formats have been developed that can be delivered on plasmid, transposon and integration-proficient vectors.
๐ SIMILAR VOLUMES
Derivatives of plasmid pRK290 that are useful for cloning and for analyzing gene expression in a wide variety of Gram-negative bacteria are described. A smaller broad host range plasmid derived from RK2, with properties similar to that of pRK290, is also described.