𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Vascular endothelial cells: Targets for studying the activity of hair follicle cell-produced VEGF

✍ Scribed by S. Lachgar; M. Charvéron; Y. Gall; J. Plouët; J. L. Bonafé

Publisher
Springer
Year
1996
Tongue
English
Weight
230 KB
Volume
12
Category
Article
ISSN
0742-2091

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✦ Synopsis

Fetal bovine aortic endothelial cells (FBAEC) were exposed to purified fractions of conditioned medium from cultures of hair dermal papilla cells (DPC) to determine the existence of any vascular endothelial growth factor (VEGF)-like paracrine activity of the latter. Such fractions were tested for stimulation of growth and migration of cultured FBAEC. In addition, VEGF secretion by DPC was measured by radioassay of VEGF receptors using FBAEC as target cells. The results showed that stimulation of FBAEC proliferation and migration following exposure to purified conditioned medium was dose-dependent. Radioreceptor assays of recombinant VEGF and purified DPC-conditioned medium showed competitive VEGF binding in FBAEC.

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