Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure–activity relationships
✍ Scribed by Ana B Garcia-Redondo; Fernanda R Roque; Marta Miguel; Rosina López-Fandiño; Mercedes Salaices
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 108 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Background:
The vasodilator properties of several peptide sequences derived from egg white proteins were screened in mesenteric resistance arteries from wistar-kyoto rats. for this, third-order branches of the mesenteric arteries from 6-month-old male rats were used. the vasodilator responses, with or without endothelium, to several peptides (0.1 mmol l(-1)) were analysed in an isometric myograph. moreover, the effect of nitric oxide (no) synthase (l-name, 100 micromol l(-1)) and cyclooxygenase (indomethacin, 10 micromol l(-1)) inhibitors on the vasodilator response was tested.
Results:
The peptides arg-ala-asp-his-pro-phe-leu, arg-ala-asp-his-pro-phe, arg-ala-asp-his-pro, tyr-arg-gly-gly-leu-glu-pro-ile-asn-phe, arg-asp-ile-leu-asn-gln and val-pro-pro showed a high endothelium-dependent vasorelaxation, whereas phe-arg-ala-asp-his-pro-phe-leu was only partially endothelium-dependent. the relaxation induced by arg-ala-asp-his-pro-phe-leu, arg-ala-asp-his-pro-phe, arg-ala-asp-his-pro, arg-asp-ile-leu-asn-gln and val-pro-pro was mainly mediated by no, since the response was inhibited only by l-name, while both l-name and indomethacin inhibited the vasodilator response induced by phe-arg-ala-asp-his-pro-phe-leu and tyr-arg-gly-gly-leu-glu-pro-ile-asn-phe. the presence of arg or tyr at the n-terminal position could be related to the vasodilator activity of these compounds in this vascular bed. the well-known angiotensin-converting enzyme inhibitor captopril showed only a slight vasodilator effect.
Conclusion:
These peptides could reduce the vascular resistance and be used as functional ingredients in the prevention and/or treatment of hypertension and other associated disorders.