VASCULAR CO-LOCALIZATION OF PROTEOLYTIC ENZYMES AND PROTEINASE INHIBITORS IN ADVANCED PERIODONTITIS

✍ Scribed by PINCHBACK, JANE S.; GIBBINS, JOHN R.; HUNTER, NEIL

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 901 KB
• Volume: 179
• Category: Article
• ISSN: 0022-3417
• DOI: 10.1002/(sici)1096-9896(199607)179:3<326::aid-path562>3.0.co;2-b

✦ Synopsis

Sections of tissue biopsies obtained from advanced, destructive periodontitis were compared with minimally inflamed periodontal tissues in relation to the distribution of type I (interstitial) collagenase. Immunohistochemistry using a highly specific antiserum showed weak staining of occasional vessels in minimally inflamed specimens but widespread reactivity, localized to the vasculature, in advanced disease. I n situ hybridization confirmed the vascular source of type I collagenase. Minimally inflamed tissues did not react with antibody to urokinase-type plasminogen activator, a potential activator of pro-collagenase, but there was a consistent strong reaction in advanced disease. Antibody to tissue inhibitor of metalloproteinase (T1MP)-1 did not react with minimally inflamed tissues, but gave intense, widespread vascular staining in advanced disease, whereas antibody to TIMP-2 produced localized connective tissue staining. These results indicate that upregulation of proteinases and inhibitors related to the vasculature is an integral component of destructive periodontitis.