Varying populations of CD59-negative, partly positive, and normally positive blood cells in different cell lineages in peripheral blood of paroxysmal nocturnal hemoglobinuria patients
✍ Scribed by Seitoku Fujioka; Teruo Yamada
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 523 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0361-8609
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✦ Synopsis
CD59-antigen expression on the surface membranes of erythrocytes, granulocytes, monocytes, lymphocytes, and platelets was determined by flow cytometry in 34 healthy controls and 17 patients with paroxysmal nocturnal hemoglobinuria (PNH). In all PNH patients, CD54negative erythrocytes accounted for >10% of the total erythrocyte population. Two erythrocyte populations (CDWnegative and normally positlve or CD5Qnegative and partly positive), three populatlons (CD59-negative, partly positive, and normally positive), and one population (CD54negative) were demonstrated in ten, six, and one patients, respectively. However, CDIQnegative granulocytes did not account for >1 O K of the total granulocytes in two patients, and one of them had only a CD59 normally positive granulocyte population. A particular granulocyte population extended over both CD54 negative and partly positive areas was shown in two patients. Two populations (CD54 negative and normally positive) and one population (CDBQnegatlve) were demonstrated in monocytes and lymphocytes. CDBQnegative lymphocytes accounted for >SO% of the total lymphocytes in only two patients. Three patients had a CD59 normally positive lymphocyte population. Percentages of CD59-positive platelet population in normal controls were widely various. Therefore, it was usually difficult to discriminate between PNH-affected and normal platelets. Thus, the flow cytometric profiles of CD54antigen expression varied not only between PNH patients but between cell lineages. The present results and our prior study indicate that CD59 flow cytometry using erythrocytes and granulocytes is most suitable for diagnosing PNH.