The abundance of mitochondrial DNA (mtDNA) deletions has been shown to increase with age in a number of species and may contribute to the aging process. Estimating the total mtDNA deletion load of an individual is essential in evaluating the potential physiological impact. In this study, we compared
โฆ LIBER โฆ
Varied Prevalence of Age-Associated Mitochondrial DNA Deletions in Different Species and Tissues: A Comparison between Human and Rat
โ Scribed by Chunfang Zhang; Margaret Bills; Anita Quigley; Ronald J. Maxwell; Anthony W. Linnane; Phillip Nagley
- Book ID
- 115579701
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 349 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0006-291X
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We have developed an improved allele-specific polymerase chain reaction (AS-PCR) procedure that can selectively amplify mutant DNA sequences (which differ from the normal sequences by a single base pair) in the presence of large excess of normal sequences. We applied this procedure to quantification