Variations in tumor cell growth rates and metabolism with oxygen concentration, glucose concentration, and extracellular pH

✍ Scribed by Joseph J. Casciari; Stratis V. Sotirchos; Robert M. Sutherland

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 912 KB
• Volume: 151
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041510220

✦ Synopsis

Tumors and multicellular tumor spheroids can develop gradients in oxygen concentration, glucose concentration, and extracellular pH as they grow. In order to calculate these gradients and assess their impact on tumor growth, it is necessary to quantify the effect of these variables on tumor cell metabolism and growth. In this work, the oxygen consumption rates, glucose consumption rates, and growth rates of EMT6IRo mouse mammary tumor cells were measured at a variety of oxygen concentrations, glucose concentrations, and extracellular pH levels. At an extracellular pH of 7.25, the oxygen consumption rate of EMTGiRo cells increased by nearly a factor of 2 as the glucose concentration was decreased from 5.5 m M to 0.4 mM. This effect of glucose concentration on oxygen consumption rate, however, was slight at an extracellular pH of 6.Y.5 and disappeared completely at an extracellular pH of 6.60. The glucose consumption rate of EMTGIRo cells increased by roughly 40% when the oxygen concentration was reduced from 0.21 m M to 0.023 m M and decreased by roughly 60% when the extracellular pH was