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Variations in the sensitivity of proteins to the protein assay procedures

โœ Scribed by Ruthven N.A.H. Lewis

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
403 KB
Volume
99
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

The variation in the sensitivity of proteins to some commonly used protein assay procedures was estimated by a calculation of the ideal titer per unit weight of protein for a sample of 350 proteins. On the basis of such calculations, the variation expected of nitrogenbased assay procedures is expected to be small, and such procedures are considered to give a more consistent quantification of a variety of proteins than other commonly used assay procedure.

2 The widely used procedures based on the use of Folin Ciocalteau phenol reagent (11) have been shown 1 Present address: Department of Biochemistry, Unito exhibit a complex stoichiometric relationship with versity of Alberta, Edmonton, Alberta T6G 2H7, protein (12). Consequently such procedures cannot be Canada.

treated as outlined in this paper.

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