The density of lipophorin was determined in individual Manduca sexta during development from the second day of the fifth larval instar to the second day of the pupal stage. Lipophorin formed defined bands when subjected to density gradient ultracentrifugation. All lipophorin observed was high density lipophorin; however, the densities varied from 1 .I 00 to 1 .I 84 g/ml, and 40% of the animals had more than one density form of lipophorin. The lipophorins were divided into five density classes: class 1 from 1 .I00 to 1.1 13 g/mi, class 2 from 1.114 to 1.132 dml, class 3 from 1.133 to 1.145 g/ml, class 4 from 1.146 to 1 .I 62 dml, and class 5 from 1 .I 63 to 1.1 84 g m l . In feeding larvae, classes 2 and 3 were the most abundant. Larvae of the first day of wandering had either lipophorin in class 2 or in classes 2 and 5. Later during wandering the variation increased, but on the third day most of the lipophorin was in class 2. In first day pupae, only lipophorins of classes 4 and 5 were detected, while on the second day of the pupal stage, classes 2 and 3 were predominant.
Class 1 lipophorin was abundant in larvae injected with Manduca adipokinetic hormone (M-AKH), and rare in young feeding larvae. In no other stage was class 1 lipophorin observed. Our results show that the density of lipophorin is much more variable than previously reported which makes it difficult to ascribe any lipophorin density to a developmental stage. These results also show that adipokinetic hormone decreases the density of lipophorin in larvae.