Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhewhich differ in their minor nucleotide content
✍ Scribed by H. Pfitzinger; L. Maréchal-Drouard; D. T. N. Pillay; J. H. Weil; P. Guillemaut
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 415 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNA Ph~ species, namely tRNAPhel and tRNAPh¢2. By sequence determination, we show that tRNAPh~2 is identical to the previously sequenced tRNAPh~I except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAs Ph* vary during leaf development: in etiolated leaves the undermodified tRNAPh*2 only represents 15 ~ of total chloroplast tRNA Ph*, during development and greening it increases to reach 60~o in 8-day-old leaves, and it then decreases to 9 ~ in senescing leaves.