Variation of chromosome numbers and essential oil components of plants derived from anther culture of the diploid and the tetraploid inPelargonium roseum
✍ Scribed by Satoru Tokumasu; Masahiro Kato
- Book ID
- 104624792
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 817 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0014-2336
No coin nor oath required. For personal study only.
✦ Synopsis
Anthers of the diploid (2n = 77) and the colchi-tetraploid (2n = 154) Pelargonium roseum were cultured m vitro. In both ploidy level anthers containing uninucleate or binucleate microspores were incubated on a modified White's medium. Calli formed were then subcultured on Murashige and Skoog's medium for organoid differentiation. Plants developed from organoids were transferred to filter paper bridges and after that transplanted into pots. Plants derived from anthers of the tetraploid had diploid chromosome number. Wide variation of their essential oil components suggested their genetic heterogeneity. Further, high correlations between different seasons in the rate of essential oil components showed that the wide variation was due to genetic differences. Therefore, these plants probably originated from pollen grains. On the other hand, plants derived from anthers of the diploid had diploid chromosome number. Small variation and low correlations between different seasons in essential oil components indicated their genetic homogeneity. Their origin was ascribable to the somatic tissues of the mother plant. It is concluded that in plant species in which usual sexual reproduction is difficult, anther culture of chromosome-doubled plants will give a useful method for obtaining genetic variation.