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Variation in thickness of the large cryosections cut for whole-body autoradiography

✍ Scribed by Tsunao Ito; A. Bertrand Brill

Publisher: Elsevier Science
Year: 1991
Weight: 644 KB
Volume: 42
Category: Article
ISSN: 0883-2889
DOI: 10.1016/0883-2889(91)90072-9

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✦ Synopsis

A method to assess variation in thickness of the large cryosections for whole-body autoradiography (WBARG) was described, and the degree of intraslice and interslice variations were determined for our cryomicrotome system (LKB PMV-2250). Intraslice variation in thickness of the 180 x 80 mm cryosection was 0.72-0.92 micron within the range of section thickness for WBARG (15-50 microns), and interslice variation was 0.89-1.21 microns. These potential variations in section thickness should be kept in mind whenever working with quantitative WBARG.

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